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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/09/2022 |
Actualizado : |
22/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. |
Afiliación : |
ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; DÉBORAH ERNST, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ANDREA CARDOZO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ALEXIA TORRES, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ROBERTO VIDAL, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
Título : |
Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 |
Descripción física : |
SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) |
ISSN : |
0719-8132 (print); e-ISSN 0719-8000 (electronic) |
DOI : |
10.4067/S0719-81322022000200045 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy |
Contenido : |
ABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. MenosABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The ... Presentar Todo |
Palabras claves : |
Antimicrobial resistance; Non-O157 STEC; PLATAFORMA EN SALUD ANIMAL; Shiga toxin subtypes. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16768/1/10.4067-s0719-81322022000200045.pdf
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Marc : |
LEADER 03084naa a2200325 a 4500 001 1063578 005 2022-09-22 008 2022 bl uuuu u00u1 u #d 022 $a0719-8132 (print); e-ISSN 0719-8000 (electronic) 024 7 $a10.4067/S0719-81322022000200045$2DOI 100 1 $aUMPIÉRREZ , A. 245 $aNon-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay.$h[electronic resource] 260 $c2022 300 $cSSN 0719-8132 (version on-line) ISSN 0719-8000 (version print) 500 $aArticle history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022. Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy 520 $aABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. 653 $aAntimicrobial resistance 653 $aNon-O157 STEC 653 $aPLATAFORMA EN SALUD ANIMAL 653 $aShiga toxin subtypes 700 1 $aERNST, E. 700 1 $aCARDOZO, A. 700 1 $aTORRES, A. 700 1 $aFERNÁNDEZ, M. 700 1 $aFRAGA, M. 700 1 $aVIGNOLI, R. 700 1 $aBADO, I. 700 1 $aVIDAL, R. 700 1 $aZUNINO, P. 773 $tAustral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
16/06/2022 |
Actualizado : |
16/06/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
GALLINO, J. P.; CASTILLO, A.; CERETTA, S.; ESTEVES, P.; BONNECARRERE, V. |
Afiliación : |
JUAN PABLO GALLINO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SERGIO EDUARDO CERETTA SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PATRICIO ESTEVES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
A simple and inexpensive procedure to more quickly obtain new varieties in soybean. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Crop Breeding and Applied Biotechnology, 2022, volume 22, Issue 1, e38212216. OPEN ACCESS. doi: https://doi.org/10.1590/1984-70332022v22n1a06 |
ISSN : |
1518-7853 |
DOI : |
10.1590/1984-70332022v22n1a06 |
Idioma : |
Inglés |
Notas : |
Article history: Received 24 May 2021; Accepted 12 Aug 2021; Published 30 Mar 2022; Publication in this collection 04 May 2022; Date of issue 2022.
Corresponding author: Gallino, J.P.; INIA, Estación Experimental "Wilson Ferreira Aldunate", Las Brujas, Ruta 48, km 10, Rincón del Colorado, Canelones, Uruguay; email:jpgallino@gmail.com -- The authors thanks the Instituto Nacional de Investigación Agropecuaria (INIA) for financial support and use of facilities.
License: This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ |
Contenido : |
ABSTRACT - Soybean (Glycine max L.) is one of the four most important crops in the world. The creation of new commercial varieties is a long-term activity that requires from seven to eight years from the beginning of the cross design up to registration for commercialization of cultivars. Rapid generation advance (RGA) is a technique that consists of controlling external factors that affect plant growth with the aim of shortening the sowing to harvest cycle. In the present study, an optimized method is described that can accelerate soybean breeding by means of RGA using inexpensive facilities and that can be easily applied by breeders. Our breeding method uses a modified cold storage chamber fitted with fluorescent lamps delivering a 12/24 h light photoperiod, while temperature is set at 24 °C. This method allows development of up to 5 generations per year instead of the 1-2 generations currently possible under field or greenhouse conditions. © 2022, Brazilian Society of Plant Breeding. All rights reserved. |
Palabras claves : |
Genetic gain; Rapid generation advance; Selection cycle; Soybean. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
https://www.scielo.br/j/cbab/a/CX3xLpkzDjsvGDkMvKxDnHh/?format=pdf&lang=en
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Marc : |
LEADER 02458naa a2200253 a 4500 001 1063312 005 2022-06-16 008 2022 bl uuuu u00u1 u #d 022 $a1518-7853 024 7 $a10.1590/1984-70332022v22n1a06$2DOI 100 1 $aGALLINO, J. P. 245 $aA simple and inexpensive procedure to more quickly obtain new varieties in soybean.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 24 May 2021; Accepted 12 Aug 2021; Published 30 Mar 2022; Publication in this collection 04 May 2022; Date of issue 2022. Corresponding author: Gallino, J.P.; INIA, Estación Experimental "Wilson Ferreira Aldunate", Las Brujas, Ruta 48, km 10, Rincón del Colorado, Canelones, Uruguay; email:jpgallino@gmail.com -- The authors thanks the Instituto Nacional de Investigación Agropecuaria (INIA) for financial support and use of facilities. License: This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ 520 $aABSTRACT - Soybean (Glycine max L.) is one of the four most important crops in the world. The creation of new commercial varieties is a long-term activity that requires from seven to eight years from the beginning of the cross design up to registration for commercialization of cultivars. Rapid generation advance (RGA) is a technique that consists of controlling external factors that affect plant growth with the aim of shortening the sowing to harvest cycle. In the present study, an optimized method is described that can accelerate soybean breeding by means of RGA using inexpensive facilities and that can be easily applied by breeders. Our breeding method uses a modified cold storage chamber fitted with fluorescent lamps delivering a 12/24 h light photoperiod, while temperature is set at 24 °C. This method allows development of up to 5 generations per year instead of the 1-2 generations currently possible under field or greenhouse conditions. © 2022, Brazilian Society of Plant Breeding. All rights reserved. 653 $aGenetic gain 653 $aRapid generation advance 653 $aSelection cycle 653 $aSoybean 700 1 $aCASTILLO, A. 700 1 $aCERETTA, S. 700 1 $aESTEVES, P. 700 1 $aBONNECARRERE, V. 773 $tCrop Breeding and Applied Biotechnology, 2022, volume 22, Issue 1, e38212216. OPEN ACCESS. doi: https://doi.org/10.1590/1984-70332022v22n1a06
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